Fig. 4
From: Comparison of DNA targeting CRISPR editors in human cells
Gene activation comparison of the DNA targeting CRISPRa systems in mammalian cells. a Schematic of the gene activation system based on DNA targeting CRISPR systems. VPR, synthetic VP64-p65-Rta activation domain. b–d qPCR analyses of the transcriptional activation levels with DNA targeting CRISPRa guided by a single sgRNA targeting each promoter region of Fgf21 mouse B16 cells (b), and IL1RN and HBG in human cells HEK293T (c) and MCF7 (d), respectively. Mean values are presented with SEM, n = 3 independent experiments