Fig. 2

Activity comparison of the DNA targeting CRISPR systems in human cells. a Schematic of the editing performance analysis by Deep-seq among the DNA targeting CRISPR editors (CRISPR-AsCas12a, CRISPR-LbCas12a, CRISPR-Un1Cas12f1, CRISPR-AsCas12f1, and CRISPR-SpCas9 systems). b The editing activities of the DNA targeting systems with twenty-one sgRNAs in HEK293T, MCF7, K562, and Jurkat cells were revealed by Deep-seq (also see Additional file 1: Fig. S7). The targeted sgRNAs for Cas12 and Cas9 share a common spacer sequence. Grey points, sites without detectable editing. c FACS revealed the editing activities of the DNA targeting systems by disruption of mNeonGreen expression in a HEK293T-KI reporter cell line. The editing efficiency (values were shown with blue) was determined as the proportion of GFP negative cells within the Cas-nucleases transfected cells (mCherry-positive). n. sgRNA3/5, sgRNAs targeting mNeonGreen. Mean values are presented with SEM, n = 3 independent experiments (also see Additional file 11: Fig. S8, another two replicates). d The proportion of the insertions and deletions induced by the DNA targeting systems within the twenty-one sites. e The proportion of the indel size induced by the DNA targeting systems within the tewnty-one sites