Fig. 7
From: Flavivirus prM interacts with MDA5 and MAVS to inhibit RLR antiviral signaling
Flavivirus prM proteins facilitate SeV replication. A EV or TBEV prM plasmids were transfected into HEK293T cells. After 24 h, cells were infected with SeV (MOI 1.0) for another 20 h, the replication of SeV was analyzed by IFA analysis with anti-SeV and -Flag antibodies. Bar, 100 μm. B–E EV and full length (FL) of TBEV prM (B–E), TBEV truncation (C, E) or flavivirus prM (D, E) plasmids were transfected into HEK293T cells. After 24 h, cells were infected with SeV (MOI 1.0 and 10). The cells were harvested after 20 h and analyzed by immunoblot (B–D) and flow cytometry analysis (E). F Empty vector, DENV, ZIKA or TBEV prM plasmids were transfected into HEK293T cells. After 24 h, cells were infected with TBEV (MOI 1.0). The supernatants were collected after 20 h and analyzed by probe qPCR.