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Fig. 2 | Cell & Bioscience

Fig. 2

From: Flavivirus prM interacts with MDA5 and MAVS to inhibit RLR antiviral signaling

Fig. 2

Flavivirus TBEV prM inhibits IFN-I production activated by RIG-I/MDA5-MAVS. A Schematic view of RIG-I/MDA5 mediated IFN-I production. B-H EV or TBEV prM, the IFNβ-Luc plasmids together with RIG-I-N (B), MDA5 (C), MAVS (D), TBK1 (E), IKKε (F), IRF-5D (G) and STING (H) were co-transfected into HEK293T cells, the luciferase activity was measured at 20 hpt. The expression of RLRs and prM were detected by immunoblot. I–K TBEV prM together with indicated RLR plasmids were co-transfected into HEK293T cells, the expression of IFNβ (I), ISG56 (J) and CXCL10 (K) were analyzed by qPCR, GAPDH was used as normalizer. Bars represent the mean of three biological replicates and all data are expressed as mean ± SE

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