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Fig. 7 | Cell & Bioscience

Fig. 7

From: Electric field stimulation boosts neuronal differentiation of neural stem cells for spinal cord injury treatment via PI3K/Akt/GSK-3β/β-catenin activation

Fig. 7

EF pre-stimulation increased neuronal differentiation of transplanted NSCs in impacted spinal cord. AC. The transplanted EGFP-NSCs with EF pre-stimulation showed increased neurite process (A, B) and βIII-tubulin/EGFP co-localization (A, C) than those NSCs with NoEF treatment D, E. The transplanted EGFP-NSCs with EF pre-stimulation showed less GFAP/EGFP co-localization than those NSCs with NoEF treatment. Scale bars: 20 μm. * P < 0.05, ** P < 0.01 and *** P < 0.001 were considered as significantly different between EF and NoEF groups. n = 5 mice in EF and n = 6 mice in NoEF group

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