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Fig. 1 | Cell & Bioscience

Fig. 1

From: Electric field stimulation boosts neuronal differentiation of neural stem cells for spinal cord injury treatment via PI3K/Akt/GSK-3β/β-catenin activation

Fig. 1

EF stimulation increased neuronal differentiation of NSCs. A. Neurospheres and monolayer culture of NSCs for EF stimulation (B). C. The 7-day EF stimulation increased MAP2 (neuronal differentiation) and decreased GFAP (glial differentiation) protein expression in NSCs. D. EF stimulation for 7, 14 and 21 days increased neuronal, and reduced glial differentiation of NSCs. E, F. Quantification of MAP2 + and GFAP + NSCs counts with/without 7, 14 and 21-day EF stimulation. G. EF stimulation improved neurites process (βIII-tubulin + process) and synapse generation (Synapsin +). H Quantification of neurites process with/without 7, 14, 21-day EF stimulation. Scale bars: 20 μm. * P < 0.05, ** P < 0.01 and *** P < 0.001 were considered as significantly different between EF and NoEF groups

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Cell & Bioscience

ISSN: 2045-3701

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