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Fig. 8 | Cell & Bioscience

Fig. 8

From: Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification

Fig. 8

Inhibition of methylation of mechanical stimulation mediated by Piezo1. A Western blot of Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 in in control and Piezo1 knock down LS174T cells before and after mechanical stimulation (n ≥ 6). B–D Statistical analysis of Suv39h1, Hdac3 and H3K9me3 protein in the control group in panel A. E–G Statistical analysis of Suv39h1, Hdac3 and H3K9me3 protein in the Piezo1-kd group in panel A. H Immunofluorescence for co-localization of H3K9me3 and AGR2 (label of goblet cells) in LS174T cells before and after mechanical stimulation; bar length: 20 μm. I Statistical analysis of H3K9me3 intensity in panel (H) (n ≥ 4). J–M Western blot and statistical analysis of Suv39h1, Hdac3, GAPDH, H3K9me3 and T-H3 in in LS174T cells before and after Yoda1 intervention (n ≥ 5). N ELISA analysis of mucin2 in LS174T cells after Yoda1 intervention (n ≥ 6)

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