Fig. 4From: Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modificationPiezo1 agonist improved mucus barrier function and ameliorated intestinal motility disorder in WAS mice. A, B Pain threshold and AWR score in the control, WAS and WAS + Yoda1 mice (n ≥ 5). C, D Colon mucus thickness and increased mucus thickness in the control, WAS and WAS + Yoda1 mice measured by horizontal Ussing chamber (n ≥ 5). E Alcian staining of control, WAS and WAS + Yoda1 mice colon; bar length: 50 μm. F The ratio of goblet cells to epithelial cells in the control, WAS and WAS + Yoda1 mice (n ≥ 6). G RT-PCR of mucin2 mRNA in the mucosa of control, WAS and WAS + Yoda1 mice (n ≥ 5). H FISH of control, WAS and WAS + Yoda1 mice colon; mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 50 μm and 20 μm. I Results of Ink propulsion test in the control, WAS and WAS + Yoda1 mice. J–L Spontaneous activities and Acetylcholine (Ach) induced responses of colonic longitudinal muscles, including MI and MI compared to baseline, in the control, WAS and WAS + Yoda1 mice (n ≥ 5)Back to article page