Fig. 1

Impaired mucus barrier function in the WAS model mice. A, B Pain threshold and AWR score in the control and WAS mice (n ≥ 6). C, D Colon mucus thickness and increased mucus thickness in the control and WAS mice measured by horizontal ussing chamber (n ≥ 6). E RT-PCR of mucin2 mRNA in the mucosa of control and WAS mice (n ≥ 6). F The ratio of goblet cells to epithelial cells in the control and WAS mice (n ≥ 6). G Immunofluorescence of mucin2 in the control and WAS mice; bar length: 50 μm. H Alcian staining of control and WAS mice colon; bar length: 50 μm. I FISH of control and WAS mice colon, mucus was stained with UEA1 (green), bacteria were detected by fluorescence in situ hybridization with general bacterial 16S probes EUB (red) and DNA stained using DAPI (blue); bar length: 50 μm and 20 μm