Fig. 4From: SPOP inhibits BRAF-dependent tumorigenesis through promoting non-degradative ubiquitination of BRAFEC- or PCa-associated SPOP mutants are defective in promoting BRAF ubiquitination. a, b Distribution of the recurrent mutation sites on the SPOP found in EC(a) or PCa b. c, e, g Western blot of WCL and co-IP samples of anti-FLAG antibody obtained from (293Â T cells transfected with the indicated plasmids. d, f, h 293Â T cells were transfected with the indicated plasmids. The WCLs were prepared and the in vivo ubiquitination assays were performed. The polyubiquitinated forms of BRAF were detected by Western blot with anti-HA antibodyBack to article page