Fig. 3
From: SPOP inhibits BRAF-dependent tumorigenesis through promoting non-degradative ubiquitination of BRAF
The SBC motif in BRAF is recognized by SPOP. a, c, e Western blot of WCL and co-IP samples of anti-FLAG antibody obtained from 293Â T cells transfected with BRAF-WT or deletion mutants. b, d, f 293Â T cells were transfected with the indicated plasmids. 24Â h after transfection, the WCLs were prepared and the in vivo ubiquitination assays were performed. The polyubiquitinated forms of BRAF were detected by Western blot with anti-HA antibody