Fig. 1

SPOP interacts with BRAF in cells. a Amino acid sequence alignment of the SBC motif in SPOP substrates. ERG, ATF2, Caprin1, and DAXX are reported SPOP substrates containing the well-characterized SBC motif (Φ-π-S–S/T-S/T; Φ: nonpolar residues, π: polar residues). b Diagram showing the RAF protein family, putative SBC motif, and two cancer-associated BRAF mutants. c–e Western blot of indicated proteins in whole-cell lysates (WCL) and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids. f Western blot of indicated proteins in WCL and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids. g Western blot of indicated proteins in WCL and co-IP samples of IgG or anti-SPOP antibody obtained from the cell extracts of Ishikawa cells. h Western blot of indicated proteins in WCL and co-IP samples of IgG or anti-BRAF antibody obtained from the cell extracts of Ishikawa cells. i Schematic representation of SPOP deletion mutants. The binding capacity of SPOP to BRAF is indicated with the symbol. j Western blot of indicated proteins in WCL and co-IP samples of anti-FLAG antibody obtained from 293 T cells transfected with indicated plasmids