Fig. 1From: Photobiomodulation improves the synapses and cognitive function and ameliorates epileptic seizure by inhibiting downregulation of Nlgn3PBM irradiance and experimental schedule for in vivo and in vitro study. A Beam profiling of PBM using a spectrophotometer. An 825Â nm laser with specific narrow wavelength was use to irradiate the SE mouse and an 850Â nm LED with a wide range of wavelengths was use to irradiate the primary hippocampal neurons. B Experimental setup for treatment with tPBM. After the hair on the head was depilated, the mouse was placed in the mouse restrainer with a hole in the upper part to fix the movement, and then an 825Â nm laser was used for irradiation for 380Â s at a distance of 4Â cm with a power of 40Â mW/cm2. C Laser transmission ratio to skin and skull were measured by power-meter detector. D Rat hippocampal primary cultured neurons were irradiated by 850Â nm LED on DIV 13. E 825Â nm laser was irradiated at 4Â h after PI injection, seizure scoring was performed on day 0, and behavior tests were performed on day 7. Then, brains were collected for RNA-sequencing, histology, IHC, western blotting, and biochemical analyses on days 0, 3, or 7Back to article page