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Fig. 7 | Cell & Bioscience

Fig. 7

From: Inhibition of PI3 kinase isoform p110α suppresses neuroblastoma growth and induces the reduction of Anaplastic Lymphoma Kinase

Fig. 7

p110α inhibition destabilizes ALK protein in neuroblastoma cells. a, b mRNA levels of ALK were detected by qPCR after p110α inhibition by shRNA or siRNA (50 nM, 48 h) in NBL-S cells (a) and KELLY cells (b), respectively. c KELLY cells were treated with different doses of alpelisib for 24 h and ALK mRNA was analyzed by qPCR. RPL19 expression was used as the internal control in a-c. Data are presented as mean ± SEM and analyzed with the Student’s t-test for statistical significance; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. d–k NBL-S and KELLY cells were incubated with the vehicle, copanlisib (0.5 µM), or alpelisib (2 µM) for 24 h, and analyzed by western blot at different time points after cycloheximide (100 µg/ml) treatment. lo Cells with p110α knockdown were treated with cycloheximide (100 µg/ml) for different time, and ALK protein levels in these samples were detected by western blot to study ALK degradation. Quantification for each experiment was performed by Image J. The regression line for ALK degradation was then generated by GraphPad Prism. p The proposed mechanism of p110α inhibition induced tumor suppression in neuroblastoma

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