Skip to main content
Fig. 6 | Cell & Bioscience

Fig. 6

From: Inhibition of PI3 kinase isoform p110α suppresses neuroblastoma growth and induces the reduction of Anaplastic Lymphoma Kinase

Fig. 6

p110α inhibition decreases ALK in neuroblastoma cells. a Kaplan–Meier analysis of overall survival probability in neuroblastoma patients, separated by the expression of PIK3CA (SEQC (seqcnb1) dataset). The cut-off value was defined by Kaplan scan in the R2 platform (“Materials and methods” section). b Expression correlation between PIK3CA and ALK in tumor samples in SEQC (seqcnb1) dataset. c-h, NBL-S and KELLY cells were treated with p110α inhibitors (copanlisib and alpelisib) and ALK inhibitors (crizotinib and NVP-TAE-684) alone or in combination. After 72 h, cell viabilities were detected by CellTiter-Glo® Luminescent Cell Viability Assay. Data were presented as mean ± SEM and analyzed with the Student’s t-test for statistical significance; *p < 0.05; **p < 0.01; ***p < 0.001. i, j ALK levels in NBL-S and KELLY cells were determined by western blot after treating with different PI3K inhibitors at indicated doses. k–m ALK levels were determined by western blot after knockdown of p110α with either shRNA or siRNA (50 nM, 48 h). β-Tubulin was used as the loading control. n ALK levels after p110α overexpression in IMR-32 cells were detected by western blot. β-Tubulin served as the loading control

Back to article page