Fig. 9

Elovl1 is expressed in hepatocytes and at low levels in cholangiocytes from mouse and human livers. A The expression of miR-125b in mouse liver (n ≥ 3) was evaluated by qPCR. For bar graphs, data are mean ± SEM of 3 replicates from CD-fed WT mice (n = 5), EtOH-fed WT mice (n = 5), CD-fed Sct^−/− mice (n = 5), and EtOH-fed Sct^−/− mice (n = 4). *p < 0.05 vs. CD-fed WT mice; ^#p < 0.05 vs. EtOH-fed WT mice. B–C Representative images for Elovl1 in liver sections from B the selected mouse groups (n ≥ 3/group), C healthy human controls (n = 8) and patients with alcoholic cirrhosis (n = 8) by immunohistochemistry. Scale bar: 100 μM Immunohistochemical quantification of Elovl1 in human liver sections Data are mean ± SEM of 5 random fields *p < 0.05 vs. controls. D–E Immunofluorescence of Elovl1 in liver sections (co-stained with HNF4α) from D the selected mouse groups (n ≥ 3/group); orig. magn. 20X, scale bar: 100 μm. E human healthy controls (n = 2) and patients with alcoholic cirrhosis (n = 2). Orig. magn. 20X, scale bar: 100 μm. White arrows indicate Elovl1 positivity. F Measurement of Cyp27a1 and Cyp7b1 genes (which regulate BA synthesis), and the BA transporters genes, Bsep and NTCP, in total mouse liver. We demonstrated increased mRNA expression of Cyp27a1, Cyp8b1, Bsep and NTCP in total liver from EtOH-fed WT mice compared to CD-fed WT mice, which were decreased in EtOH-fed Sct^−/− mice compared to EtOH-fed WT mice. Data are mean ± SEM of three qPCR reactions from mouse total liver samples from CD-fed WT mice (n = 5), CD-fed Sct^−/− mice (n = 3), EtOH-fed WT mice (n = 5), and EtOH-fed Sct^−/− mice (n = 5) for Cyp27a1, Cyp8b1 mRNA expression, and CD-fed WT mice (n = 4), CD-fed Sct^−/− mice (n = 4; 6 experiments), EtOH-fed WT mice (n = 5), and EtOH-fed Sct^−/− mice (n = 4) for Bsep and NTCP mRNA expression. G By immunofluorescence for Ostα/CK19 and Ostα/HNF4a, there was increased immunoreactivity of Ostα in EtOH-fed WT mice compared to CD-fed WT mice, which decreased in EtOH-fed Sct^−/− mice compared to EtOH-fed WT mice. The selected mouse groups (n ≥ 3/group); orig. magn. 20X, scale bar: 100 μm. White arrows indicate Ostα positivity.