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Fig. 10 | Cell & Bioscience

Fig. 10

From: Knockout of secretin ameliorates biliary and liver phenotypes during alcohol-induced hepatotoxicity

Fig. 10

Alcohol-induced liver angiogenesis is deceased in EtOH-fed Sct−/− mice. A Paraffin-embedded liver sections were stained with VEGF-A, CD31, Robo1, and Slit2 to evaluate liver angiogenesis in the selected mouse liver (n ≥ 3/group). Orig. magn. 20X, scale bar: 100 μM. B The immunoreactivity of Robo1 and vWF was evaluated in mouse liver sections co-stained with CK19 (n ≥ 3) by immunofluorescence. Orig. magn. 20X, scale bar: 100 μM. C The immunoreactivity of VEGF-A, CD31, Robo1, and Slit2 was examined in paraffin-embedded liver sections from human control (n = 8) and patients with alcoholic cirrhosis (n = 8) by immunohistochemistry. Orig. magn. 20X, scale bar: 100 μm Immunohistochemical quantification of VEGF-A, CD31 and Robo1 in human liver sections. Evaluation of Robo1 staining data is mean ± SEM of 5 random fields *p < 0.05 vs. Control. D mRNA expression of vWF was evaluated in total liver from healthy controls (n = 6) and patients with alcoholic cirrhosis (n = 16) by qPCR. For bar graphs, data are mean ± SEM, *p < 0.05 vs. relative controls

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