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Fig. 1 | Cell & Bioscience

Fig. 1

From: Knockout of secretin ameliorates biliary and liver phenotypes during alcohol-induced hepatotoxicity

Fig. 1

A Sct, SR, and CFTR immunoreactivity significantly increased in EtOH-fed WT mice compared to CD-fed WT mice. The immunoreactivity of SR and CFTR decreased in EtOH-fed Sct−/− mice compared to EtOH-fed WT mice. Representative immunohistochemical images for Sct, SR, and CFTR in liver sections from CD-fed WT mice (n = 6), CD-fed Sct−/− mice (n = 4), EtOH-fed WT mice (n = 5), and EtOH-fed Sct−/− mice (n = 3) are shown. Orig. magn., 20X, scale bar: 100 μm. Immunohistochemical quantification of Sct and SR in mouse liver sections. Data are mean ± SEM of 5 random fields *p < 0.05 vs. CD-fed WT mice; #p < 0.05 vs. EtOH-fed WT mice. Each dot represents one value in data set. Black arrows indicate bile ducts, whereas black star symbols indicate bile ducts negative for Sct. B Sct mRNA expression was similar between CD-fed and EtOH-fed WT mice; Sct mRNA expression was absent in CD-fed and EtOH-fed Sct−/− mice. There was increased SR mRNA expression in EtOH-fed WT compared to CD-fed WT mice, which decreased in EtOH-fed Sct−/− mice compared to EtOH-fed WT mice. Data are mean ± SEM of three PCR reactions from cholangiocytes from CD-fed WT mice (n = 6), CD-fed Sct−/− mice (n = 4), EtOH-fed WT mice (n = 5), and EtOH-fed Sct−/− mice (n = 3). *p < 0.05 vs. the values of CD-fed WT mice. #p < 0.05 vs. EtOH-fed WT mice

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