Fig. 7
Ebselen suppresses colorectal cancer tumor growth. A A brief diagram of the animal experiment for subcutaneous xenografted tumor model. 2 × 106 (2e6) SW620 cells were xenografted into BALB/c-nu/nu mice by subcutaneous injection (s.c.). After 10 days, mice were injected intraperitoneally (i.p.) with indicated doses of Ebselen every two days, and monitoring of tumor volume and body weight changes were performed. B–D Representative tumor images and tumor weight at the end time point were captured from SW620 cell-based xenografts. Mean tumor weight ± SEM (C) and mean tumor volume ± SEM (D) were shown. E The inhibitory effect of ATG4 in xenografted tumors was detected by FRET assay. F, I The protein level of autophagy and apoptosis in xenografted tumors were detected by western blot. Representative results were shown and the statistical results of each group were obtained from seven mice. J, K The immunohistochemistry analysis of Ki67 in xenografted tumors (group vehicle and Ebselen 10 mg/kg). Bar = 20 μm. The positive Ki67 area rate was obtained from the ratio of positive Ki67 area to total cell area by Image J