Fig. 6

Ebselen suppresses the growth of CRC cells via ATG4B inhibition. A Bioinformatics analysis of ATG4B expression between normal (N = 41) and CRC tumor (N = 288) from the TCGA database. B Comparison of ATG4B expression between normal (N = 41) and different grades CRC tumor (grade1 N = 8, grade2 N = 40, grade3 N = 54, grade4 N = 4) from the TCGA database. C Survival analysis of ATG4B expression between high (N = 88) and low (N = 335) expression level from the TCGA database. D WT and ATG4B KO HCT116 cells were detected by colony formation assay. E The results of western blot validation of WT and ATG4B KO HCT116, RKO and SW620 cells. F–H WT and ATG4B KO HCT116, SW620 and RKO cells were treated with different concentrations of Ebselen and cell viabilities were detected by CCK-8 assay. (I) Cell numbers counting of HCT116 cells were treated with different concentrations of Ebselen. J HCT116 cells were treated with Ebselen and detected by colony formation assay