Fig. 4

Loss of interpolar microtubules in ADD1-depleted oocytes. A Immunofluorescence images of the MII spindle in calcium-treated control and ADD1-depleted mouse oocytes. Green, α-tubulin; blue, DNA; Merge, overlapping of green and blue. Enlarge, an enlarged view of the image in the white boxes of the merged image. Bar, 20 μm. B Quantification of total fluorescence intensity (arbitrary units) of spindle microtubules in calcium-treated control and ADD1-depleted mouse oocytes at the MII stage. In each set of experiments, the fluorescence intensity of spindle microtubule staining was normalized to the value of oocytes in the control group. Bars that do not share the same uppercase letter are significantly different at p < 0.01 by the student’s t-test