Fig. 2

Expression and subcellular localization of p-ADD1 during mouse oocyte meiotic maturation. A The protein levels of p-ADD1 in oocytes at various stages were analyzed by western blot. Protein loading was verified by the detection of β-actin. The molecular mass of target proteins is indicated on the right. B p-ADD1 was quantified for three independent repeats (normalized to β-actin, arbitrary units). Bars that do not share the same lowercase letter are significantly different at p < 0.05 by one-way ANOVA with the Tukey test. C Immunofluorescent localization of p-ADD1 and spindle microtubules in meiotic oocytes at various stages. D Distribution of p-ADD1 in MI and MII oocytes treated with taxol. E Localization of p-ADD1 in MI and MII oocytes exposed to nocodazole. Pink or red, p-ADD1; green, α-tubulin; blue, DNA; Merge, overlapping of pink/red, green, and blue. Bar, 20 μm