Fig. 1

Expression and subcellular localization of ADD1 during mouse oocyte meiotic maturation. A The protein levels of ADD1 in oocytes at the GV, GVBD, MI, ATI, and MII stages were detected by immunoblotting. Protein loading was verified by the detection of β-actin. The molecular mass of target proteins is indicated on the right. B ADD1 was quantified for four independent repeats (normalized to β-actin, arbitrary units). Different lowercase letters above columns denote statistical difference at p < 0.05 by one-way ANOVA with the Tukey test. C Spatial distribution of ADD1 and spindle microtubules in oocytes at different stages. D Colocalization of ADD1 and γ-tubulin in MI and MII oocytes. E Localization of ADD1 in MI and MII oocytes exposed to taxol. F Distribution of ADD1 in MI and MII oocytes treated with nocodazole. Red, ADD1; green, α-tubulin; blue, DNA; pink, γ-tubulin; Merge, overlapping of red, green, blue, and pink. Bar, 20 μm