Fig. 6

Shortened body length and abnormal cartilage development in shox and wrn knockdown zebrafish morphants. a Quantification of the body length of zebrafish larvae in injection control and morphant groups from 3 to 7 dpf. For each group, n ≥ 13. b Representative images of the standard control, shox and wrn knockdown morphants from 3 to 7 dpf. c Ventral and lateral views of the 7 dpf control, shox and wrn knockdown morphants stained with Alcian blue solution. Some larvae showed defect of skeletal structure including abnormal development of ceratohyal (ch) in shox MO larvae and breakage in Meckel’s cartilage (mk) in wrn MO larvae (filled arrow). ch, ceratohyal; mk, Meckel’s cartilage; ep, ethomoid plate; MO, Morpholino. Scale bar: 200 µm. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005. ns not significant