Fig. 4
From: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells
Helicase activity of WRN is required for the rescue of chondrogenic defect in WRN−/− MSC by restoring SHOX expression. a Western blot analysis of WRN+/+ and WRN−/− MSC rescued by expressions of WT or different mutant forms of WRN proteins. b Western blot analysis of MSC as additional controls: SHOX KO in WRN+/+ MSC and SHOX OE in WRN−/− MSC. c Alcian blue staining and immunofluorescent staining for chondrogenic markers (SOX9, COL2) in D14 chondrogenic pellets. Expression of WRN and SHOX proteins were also shown. N = 3. Scale bar: 100 µm. d RT-qPCR analysis of SOX9, COL2 and SHOX transcripts at D0, D4 and D14 during chondrogenesis. N = 3