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Fig. 3 | Cell & Bioscience

Fig. 3

From: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells

Fig. 3

Impaired chondrogenic differentiation in WRN KO and SHOX KO hESC. a Western blot analysis of WRN protein in two WRN−/− hESC clones showed null WRN expression. b Schematic diagram of directed hESC differentiation to chondrogenic colonies. c Multiple alignments for exon 2/3 of WT SHOX (NM000451) to SHOX−/− and SHOXhypo in the gene-edited hESC clones. Variations in protein sequence compared with WT SHOX protein were highlighted in orange. Premature translational terminations were highlighted in red. d Safranin-O staining of D18 chondrocytes differentiated from various mutants. N = 3. Scale bar: 500 µm. e Quantification of GAG amount in D18 chondrocytes of different mutant cells using DMMB assay. N = 3. t-test (2-tailed, unequal variance) on average of both clones compared to WT: **p < 0.005, ***p < 0.0005, ****p < 0.00005. f qPCR analysis showing gene expression relative to GAPDH (dCT) of different mutant cells at PSC, D3, D5, D9 and D18 of differentiation. N = 3. g Representative images of immunofluorescent staining in D18 chondrocytes. N = 3. Scale bar: 50 µm

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