Fig. 3

Cell-type discrimination by sciEM single nuclei DNA methylation. a Single nuclei DNA methylation clustering (NMF-tSNE). Clusters (n = 19) are defined by unique colors. b Heatmap of summarized CpG methylation z-score’s of clusters across annotated Neuron and Non-neuronal DMR’s. c Heatmap of summarized CpG methylation z-score’s of non-neuronal cell clusters across annotated non-neuronal DEG’s. d Heatmap of summarized CpG methylation z-score’s of neuronal clusters across annotated neuronal subtype CpG DMR’s. e Boxplots of CpG, CHG and CHH DNA methylation of each cell-type. Ast astrocyte, Exc excitatory neuron, End endothelial cells, Inh inhibitory neuron, Mic microglia, Olig oligodendrocyte, OPC oligodendrocyte precursor cells, Per pericyte