Fig. 1

METTL3 plays an oncogenic role in CC. Supplementary Information The mRNA expression of METTL3 in GEO (GSE39001 and GSE63514) and GENT2 CC cohorts. The three lines inside the violin plots are the first quartile, median and third quartile. B Representative IHC staining images of METTL3 protein from CC tissues and paired adjacent non-tumor tissues (n = 8, left) and quantitatively analyzed (right). C, GSEA plot of cellular process in TCGA-CC samples. D, E The mRNA(D) and protein (E) levels of METTL3 in CC cell lines (HeLa, C-33A, SiHa and CaSki) and normal cervical epithelial cells (S12). F MTT assay displaying the effect of METTL3 on cell proliferation in HeLa and SiHa cells. G, Transwell assays were used to examine the migratory and invasive abilities of HeLa and SiHa cells with overexpression and knockdown of METTL3. H Colony formation assay of HeLa and SiHa cells with overexpression and knockdown of METTL3. I-K, Stable METTL3-silenced and control HeLa cells were subcutaneously implanted into mice. The tumor sizes were continuously recorded to draw tumor growth curves (J). The tumors were collected at the endpoint of the xenograft models and tumor weights were measured (K). L, M Metastatic tumor foci in lung were quantified (L) and confirmed by HE staining (M). All experiments were performed at least 3 independent times, and data are presented as means ± SD except where otherwise specified. *P < 0.05, **P < 0.01, ***P < 0.001