Fig. 4

PROTAC attenuates renal fibrosis and inflammation and enhances peritubular capillaries density of UUO mice. A Experimental design of evaluating the therapeutic effect of PROTAC on UUO animal models. B Western blot analyses of HIF-1α, HIF-2α, Smad3 and \(\alpha\)-SMA expression and C quantification of them in UUO mice (n = 6/per group). D Representative Masson’s trichrome staining, F4/80 and CD34 immunochemical staining of renal sections among indicated groups. E The positive-stained area of Masson’s trichrome was quantitatively measured (\(\times\)400 magnification, n = 6/per group). F The count of F4/80⁺ macrophages/HPF of indicated groups (n = 6/per group). G The count of CD34⁺ capillary/HPF of indicated groups (n = 6/per group). The serum level of H VEGF was determined in indicated groups (n = 4–6/per group). Veh = vehicle, i.p. = intraperitoneal, UUO = unilateral ureteral obstruction, IHC = immunohistochemistry, HPF = high power field (magnification: \(\times\)400). Scale bar = 500 μm. Data are means ± SD, NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001