Fig. 8

FOXO3a and PARKIN formed regulatory axis of mitophagy and hypertrophy. A and B, FOXO3a inhibited cardiomyocyte hypertrophy through targeting PARKIN. After being infected with FOXO3a adenovirus, cardiomyocytes were infected with PARKIN siRNA or PARKIN scramble, and then treated with Ang II. A Cell surface area indicated by phalloidin-TRITC conjugate stained F-actin were calculated. * p < 0.05. ** p < 0.01. n = 3 experiments per group. B The mRNA levels of ANP and BNP were detected. * p < 0.05. n = 3 experiments per group. C, FOXO3a regulated mitophagy through targeting PARKIN. After being infected with FOXO3a adenovirus, cardiomyocytes were infected with PARKIN siRNA or PARKIN scramble, and then treated with Ang II. Immunoblot were performed to detect protein levels of LC3I and LC3II. The ratio of LC3II/LC3I was calculated. ** p < 0.01. n = 3 experiments per group