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Fig. 2 | Cell & Bioscience

Fig. 2

From: FOXO3a-dependent PARKIN negatively regulates cardiac hypertrophy by restoring mitophagy

Fig. 2

PARKIN inhibited Ang II-induced cardiomyocyte hypertrophy. A, Immunoblotting results exhibiting the protein levels of PARKIN in cardiomyocytes treated with Ang II at the indicated time. B, Protein levels of PARKIN in cardiomyocytes infected with Parkin adenovirus or β-gal adenovirus. C, Protein levels of PARKIN in cardiomyocytes infected with PARKIN siRNA or PARKIN scramble. D − F, Overexpression of PARKIN suppressed Ang II-induced cardiomyocyte hypertrophy. Cardiomyocytes infected with Parkin adenovirus or β-gal adenovirus were exposed to Ang II. (D) Sarcomere organization stained with phalloidin-TRITC conjugate; bar = 20 µm. Blue represent nucleus. Red represent F-actin. (E) Cell surface was calculated. ** p < 0.01. *** p < 0.001. n = 3 experiments per group. (F) The mRNA levels of ANP and BNP were analyzed by qRT-PCR. The results were normalized to GAPDH. * p < 0.05. **p < 0.01. *** p < 0.001. n = 3 experiments per group. G − I, Knockdown of PARKIN induced hypertrophic responses. Cardiomyocytes were infected with PARKIN siRNA or PARKIN scramble. G Sarcomere organization stained with Phalloidin-TRITC conjugate; bar = 20 µm. Blue represent nucleus. Red represent F-actin. H Cell surface was calculated. * p < 0.05. n = 3 experiments per group. I The mRNA levels of ANP and BNP were analyzed by qRT-PCR. The results were normalized to GAPDH. * p < 0.05. n = 3 experiments per group

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