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Fig. 6 | Cell & Bioscience

Fig. 6

From: Aurora kinase a promotes the progression of papillary thyroid carcinoma by activating the mTORC2-AKT signalling pathway

Fig. 6

SIN1 mediates AURKA functions in regulating PTC progression. A The protein expression levels of SIN1 and P-AKT were examined by western blotting in OE AURKA-transfected KTC cells and siSIN1/OE AURKA-transfected KTC cells. Mg132 (20 μM) was added 6 h prior to cell harvest for WESTERN BLOT. B KTC cells were stably transduced with AURKA or siSIN1 were cultured for 14 days prior to crystal violet staining. C Transwell migration assays were carried out using KTC cells stably overexpressing AURKA and siSIN1-transfected KTC cells or the corresponding negative control (NC) cells. D The ratio of EdU-labelled cells was observed in AURKA-overexpressing KTC cells and siSIN1-transfected KTC cells compared with the control group. E, F Representative images of subcutaneous xenografts in nude mice derived from OE AURKA-transfected KTC cells or PCDH-transfected KTC cells or shSIN1/OE AURKA-transfected KTC cells. G The expression levels of AURKA, Ki67, P-AKT and SIN1 in xenografts of each group were assessed by immunohistochemical staining. The scale bar is 50 μm. Data are shown as the mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001)

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