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Fig. 3 | Cell & Bioscience

Fig. 3

From: Aurora kinase a promotes the progression of papillary thyroid carcinoma by activating the mTORC2-AKT signalling pathway

Fig. 3

AURKA activates AKT kinase in PTC. A Volcano plot of the RNA-seq analysis results for TPC-1 cells after transfection with shAURKA. B Representative KEGG pathway analysis and GO term analysis of upregulated and downregulated genes after transfection with shAURKA. C GSEA showed that genes differentially expressed after AURKA knockdown were significantly enriched in the gene sets related to the P13K/AKT signalling pathway and adhesion. D Western blotting validation of PI3K/AKT signalling pathway component expression in AURKA knockdown TPC-1 cells and AURKA-overexpressing KTC cells. E AURKA overexpression plasmid-transfected KTC cells were maintained in medium containing an AKT inhibitor for 48 h, and then the levels of P-AKT were detected by western blotting. F KTC cells overexpressing AURKA were maintained in medium containing an AKT inhibitor for 48 h, and cell viability was determined using a CCK-8 kit. G&H AURKA-overexpressing KTC cells were maintained in medium containing an AKT inhibitor for 48 h, followed by clonogenic and transwell migration assays. Data are shown as the mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001)

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