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Fig. 2 | Cell & Bioscience

Fig. 2

From: In vivo genome-wide CRISPR screening identifies ZNF24 as a negative NF-κB modulator in lung cancer

Fig. 2

Ectopic expression of ZNF24 induces cell-cycle arrest of lung cancer cell. A Impact of ZNF24 expression on cell cycle in A549i cells. A549i cells were treated with Dox (1 μg/mL) for 48 h. Cell cycle were determined through FACS analysis of DNA contents revealed by propidium Iodide (PI) staining. Results are represented as percent of cell population in G0/G1, S and G2/M phases of the cell cycle. B Impact of ZNF24 expression on cell cycle of Hop62i cells. Hop62i cells were treated with Dox (1 μg/mL) for 48 h. Propidium iodide (PI)-stained cells were analyzed by FACS. C Impact of ZNF24 expression on proliferation of A549i cells. A549i cells were cultured in media containing 1 μg /mL of Dox for 48 h. Edu dye was administered at 10 μM. DNA replication was detected by Edu incorporation through fluorescent microscopy. Scale Bar = 200 μm. D Impact of ZNF24 expression on division proliferation of Hop62i cells. Hop62i cells were cultured in media containing 1 μg/mL of Dox for 48 h. Edu dye was administered at 10 μM. DNA replication was detected by Edu incorporation through fluorescent microscopy. Scale Bar = 200 μm. E Effect of ZNF24 expression on division proliferation of A549i cells. Cells were stained with 5 μM of CFSE Dye, followed by culture in media containing 1 μg/mL of Dox for 48 h. Cell proliferation as reflected by serial dilution of fluorescence intensity was analyzed by flow cytometry. F Statistics of (E). G Effect of ZNF24 expression on division proliferation of Hop62i cells. Cells were stained with 5 μM of CFSE Dye, followed by culture in media containing 1 μg /mL of Dox for 48 h. Cell proliferation as reflected by serial dilution of fluorescence intensity was analyzed by flow cytometry. H Statistics of (G). I Impact of ZNF24 expression on cell cycle related genes. The A549i cells were induced with Dox for 48 h and RNA was extracted. Expression of genes encoding CDK2, Cyclin D1 and Cyclin E1 was quantified through RT-qPCR. J Impact of ZNF24 expression on cell cycle related genes. The Hop62i cells were induced with Dox for 48 h for RNA extraction. Expression of genes encoding CDK2, Cyclin D1 and Cyclin E1 was quantified through RT-qPCR. KN Impact of ZNF24 on expression of cell cycle-associated proteins in A549i, Hop62i, EKVX and H322 cells through Western blot analysis

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