Fig. 4

TSP2 shRNA-AAV treatment ameliorated the neurite and synaptic alteration, and the degenerated visual function following RI/R. A Immunofluorescence images showed the morphological alteration of RGCs axons with TSP2 shRNA-AAV treatment following RI/R. B, C Bar graphs depicted the number of fascicles per view and the number of pNF + RGC axons per fascicle in each group (n = 6). D Visual function following RI/R with TSP2 shRNA-AAV treatment were measured by f-VEP test. E, F Bar graphs depicted the amplitude of N2-P2 and the latency of P2 in each group (n = 10). G Western blotting showed the change in expression of synaptophysin (SYN), synapsin 1 and homer 1b/1c in rat retinae with TSP2 shRNA-AAV treatment following RI/R. H, J Bar graphs depicted the fold of optical density value (ODV) of SYN, synapsin 1 and homer 1b/1c in each group (n = 6). K Double-immunofluorescence images showed spatial location and origin of SYN in each group with TSP2 shRNA-AAV treatment following RI/R. Data in (B, C, E, F, H, J) were represented as mean ± SD; *p < 0.05, **p < 0.01, n.s.: no significance, (compared with the RI/R group using one-way analysis of variance). Bar = 50 μm in (A, K). The abbreviations are as defined in Fig. 1