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Fig. 3 | Cell & Bioscience

Fig. 3

From: Astrocyte derived TSP2 contributes to synaptic alteration and visual dysfunction in retinal ischemia/reperfusion injury

Fig. 3

Thrombospondin2 (TSP2) shRNA-adeno-associated virus (AAV) treatment suppressed the expression of TSP2 in rat retinae following RI/R. A Western blotting showed the change of TSP2 expression in rat retinae following RI/R. B Double-immunofluorescence images revealed the origination of TSP2. C Bar graphs depicted the fold of optical density value (ODV) of TSP2 in each group (n = 6). D Western blotting showed the change of TSP2 expression in rat retinae with the FC treatment following RI/R. E Bar graphs depicted the fold of optical density value (ODV) of TSP2 in each group compared to I/R group (n = 5). F Diagram of TSP2 shRNA AAV treatment before RI/R. G Fluorescence images depicted the transduction of AAV following intravitreal injection of AAV-GFP-TSP 2 shRNA. Co-staining with GFAP revealed that the TSP2 shRNA was expressed in astrocytes in rat retina (shown by the white arrows). H QPCR showed the change of TSP2 mRNA level in rat retinae with the TSP2 shRNA-AAV treatment following RI/R (n = 6). I Western blotting showed the change of TSP2 expression in rat retinae with the TSP2 shRNA-AAV treatment following RI/R. J Bar graphs depicted the fold of optical density value (ODV) of TSP2 in each group compared to I/R group (n = 6). K Western blotting showed the change of GFAP expression in rat retinae with the TSP2 shRNA-AAV treatment following RI/R. L Bar graphs depicted the fold of optical density value (ODV) of GFAP in each group (n = 6). Data in (C) were represented as mean ± SD; *** p < 0.001, (compared with the control group using one-way analysis of variance). Data in (E, J, L) were represented as mean ± SD; ***p < 0.001, n.s.: no significance, (compared with the RI/R group using one-way analysis of variance). Bar = 50 μm/20 μm in (B), Bar = 100 μm in (G). The abbreviations are as defined in Fig. 1

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