Fig. 6

Activation of Piezo1 decreases renin expression and blood pressure in vivo. A Schematic design of the animal experiments. B qRT-PCR results of the mRNA expression of Piezo1 in kidneys from AAV-shControl (n = 3) and AAV-shPiezo1 (n = 4) mice (37 days post infection). C Multiplexed immunohistochemical staining results showing that Piezo1 expression and distribution after AAV infection in mouse kidney. Scale bar = 50 µm. D Immunohistochemical staining showing the changes of renin expression in day 12 post Veh/Yoda1/Captopril treatment after AAV infection in mouse kidney (Veh, n = 3, Yoda1, n = 4, Captopril, n = 4). Scale bar = 1000 µm. E Quantification of the expression of renin in different treatment groups by the average optical density (AOD) values of IHC. ns no significant; ** p < 0.01. Data are represented as mean ± SEM. F The levels of systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP), and heart rates of mice in day 0 and day 12 post Veh/Yoda1/Captopril treatment, (Veh, n = 3, Yoda1, n = 4, Captopril, n = 4). ns no significant; **p < 0.01; ***p < 0.001; ****p < 0.0001. Data are represented as mean ± SEM