Fig. 5

Piezo1 regulates renin expression through Ptgs2 (COX-2). A and B Quantification of the mRNA and Protein expression level of Ptgs2 (COX-2) in WT and Piezo1-KO As4.1 cells with or without Yoda1 (20 µM) treatment (n = 3). C and D Quantification of the fold changes in mRNA (C) and secreted renin (D) levels in WT and Piezo1-KO As4.1 cells with Yoda1 (20 µM) treatment in the presence or absence of NS398 (a COX-2 inhibitor) by qRT-PCR and ELISA, respectively (n = 3). E and F Quantification of the fold changes of the secreted PGE₂ and cAMP levels in WT and Piezo1-KO As4.1 cells treated with Yoda1 (20 µM) with or without NS398 (n = 3). ns no significant; *p < 0.05; **p < 0.01; ****p < 0.0001. Data are represented as mean ± SEM