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Fig. 3 | Cell & Bioscience

Fig. 3

From: Activation of Piezo1 downregulates renin in juxtaglomerular cells and contributes to blood pressure homeostasis

Fig. 3

Piezo1 contributes to the mechanosensation in As4.1 cells. A Schematic diagram of the flow-induced MS loading system. Red arrows indicate the flow-induced MS direction. B Representative images of calcium imaging assay in As4.1 cells before and after MS treatment (30 dyn/cm2). White arrows indicate the cells responding to the MS. Scale bar = 20 µm. C Representative traces of the [Ca2+]i changes in WT As4.1 cells in response to repeated MS (20 dyn/cm2, n = 3). D Quantification of the AUC of the [Ca2+]i increase from (C). E Representative traces of [Ca2+]i changes in WT and Piezo1-KO As4.1 cells in response to the MS (10 dyn/cm2, n = 3). F Quantification of the AUC of the [Ca2+]i increase from (E). G Quantification of the fold changes in mRNA level in WT and Piezo1-KO As4.1 cells in response to the MS (20 dyn/cm2) treatment by qRT-PCR. (n = 3). *p < 0.05; **p < 0.01; ****p < 0.0001. Data are represented as mean ± SEM

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