Fig. 2
Knockout of Piezo1 abolishes the effect of Yoda1 on renin expression. A–C Validation of the knockout efficiency of Piezo1 by qRT-PCR (A), immunofluorescence (B), and Western blotting (n = 3 each) (C) in Piezo1-KO As4.1 cells. Scale bar = 20 µm. D Calcium imaging assay showing the [Ca2+]i increase induced by Yoda1 (20 µM) in WT and Piezo1-KO As4.1 cells (n = 3). E Peak values of the [Ca2+]i responses induced by Yoda1 from (D). F and G Quantification of the fold changes in mRNA (F) and secreted renin G levels in WT and Piezo1-KO As4.1 cells after Yoda1 (20 µM) treatment by qRT-PCR and ELISA, respectively (n = 3). H Renin mRNA basal expression in WT and Piezo1-KO As4.1 cells. (n = 5). *p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001. Data are represented as mean ± SEM