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Fig. 1 | Cell & Bioscience

Fig. 1

From: Activation of Piezo1 downregulates renin in juxtaglomerular cells and contributes to blood pressure homeostasis

Fig. 1

Piezo1 is functionally expressed in JG cells. A Screening of the cation channels in As4.1 cells by qRT-PCR. (n = 5). B Immunostaining of Piezo1 and Plasma Membrane Calcium ATPase (PMCA, a membrane marker) in As4.1 cells. Scale bar = 20 µm. C Multiplexed immunohistochemical staining of Piezo1, renin, and αSMA showing the localization in mouse kidney. Scale bar = 50 µm. White arrows indicate the JG cells; white circles indicate the glomerulus (G). D and E Calcium imaging assay showing the [Ca2+]i increase induced by different concentrations of Yoda1 in As4.1 cells. Data are displayed as dose–dependent curves and fitted with Hill equation, indicating the EC50 of Yoda1 (n = 4). F Calcium imaging assay showing the effect of RR (Ruthenium Red, a cation channel blocker) on Yoda1 induced [Ca2+]i increase in As4.1 cells (n = 4). G Calcium imaging assay showing the [Ca2+]i increase induced by Yoda1 in the presence or absence of extracellular Ca2+ in As4.1 cells (n = 4). H and I Calcium imaging assay showing the [Ca2+]i increase induced by Yoda1 while changing the extracellular solution with or without Ca2+ in As4.1 cells (n = 4). All data are represented as mean ± SEM

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