Fig. 7

Piezo1 is activated by mechanical loading and induces the opening of Cx43 HCs in vivo. A and B 15-week-old male C57BL/6 J mice were treated with M1, IgG, or/and Yoda1, or vehicle DMSO and Evan blue dye. Representative images of tibia frozen sections of Evans blue uptake (A) and quantification of Evans blue intensity (B), n = 5 per group; C and D 15-week-old male C57BL/6 J mice were treated with Cx43(M1), IgG, or/and Dooku1, or DMSO and tibia compression and Evan blue dye. Representative images of Evans blue uptake (C) and quantification of Evans blue intensity (D). n = 5 per group. Data are shown as mean ± SEM, *** p < 0.001, ****p < 0.0001; White arrows represent osteocytes uptaking Evans Blue. Statistical analysis was performed using One-way ANOVA for multiple comparison analysis for panel B and Two-way ANOVA between multiple groups with two variables for panel D