Fig. 4

Extracellular Ca²⁺ and P2X receptor signaling are involved in Piezo1 induced elevated intracellular Ca²⁺ signals. A and B Ca²⁺ signals and AUC of MLO-Y4 cells pre-incubated with BAPTA with/without additional application of BAPTA at 120 s after application of Yoda1. n = 8, 5, and 3 for control, 1xBAPTA, and 2xBAPTA group, respectively. C and D Ca²⁺ signals and AUC of MLO-Y4 cells under a low extracellular Ca²⁺ environment, followed with Yoda1 treatment, n = 8 and 3 for control and low extracellular Ca²⁺ environment group, respectively. E and F Ca²⁺ signals and AUC of MLO-Y4 cells pre-incubated with oATP, followed by Yoda1 treatment, n = 5 and 3 for control and oATP group, respectively. G and H Ca²⁺ signals and AUC of MLO-Y4 cells pre-incubated with apyrase, followed with Yoda1 treatment. n = 5 and 3 for control and apyrase group, respectively; Data are shown as mean ± SEM, *p < 0.05, **p < 0.01. Student-Newman Keul’s test for panels D, F, and H, and One-way ANOVA was used in panel B