Fig. 1

Piezo1 agonist Yoda1 induces the opening of Cx43 HCs in a dose and time-dependent manner. A Time-lapse EtBr dye uptake level in MLO-Y4 cells (5 min interval) with different concentrations of Yoda1 (10, 15, 20, and 25 μM) treatment. B Time-lapse EtBr dye uptake level in MLO-Y4 cells (5 min interval) pre-incubated with 2 μg/mL Cx43(E2) antibody or 100 μM carbenoxolone (CBX), followed by 20 μM Yoda1 treatment. C EtBr dye uptake level at 30 min after Yoda1 application in (B). D EtBr dye uptake level in MLO-Y4 cells pre-incubated with 2 μg/mL Cx43(E2) antibody, followed by different concentrations of Yoda1 (15, 20, and 25 μM) stimulation. E Representative images showing EtBr dye uptake in Rosa26 and Cx43 KD MLO-Y4 cells pre-incubated with 2 μg/mL Cx43(E2) antibody, followed by treatment with varying concentrations of Yoda1 (15, 20, and 25 μM), scale bar = 20 μm. F Quantification of EtBr dye uptake level (E). Data are shown as mean ± SEM. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Statistical analysis was performed using One-way ANOVA for multiple comparison analysis in panel C and Two-way ANOVA with post hoc Tukey to assess the difference between multiple groups in panels A, B, D, and F