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Fig. 4 | Cell & Bioscience

Fig. 4

From: Defb19 regulates the migration of germ cell and is involved in male fertility

Fig. 4

Knockout of Defb19 leads to male subfertility. a Representative immunofluorescence staining images of paraffin-embed testis section showing the protein expression of DEFB19 (green) in WT and KO mice (n = 5 mice). Sertoli cells were marked by SOX9 (red) and nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. b Representative images of the testis and the epididymis of KO and WT mice. c Tissue to body weight ratio of the testis and epididymis of KO and WT mice (n = 4 mice). d, e Number of sperm isolated from the epididymis and vas deferens (e, n = 6 mice) and the motility of sperm (e, n = 5 mice) in WT and KO mice. f Quantitation of the number of litters sired by WT females after cross breeding with KO or WT male. Data are presented as means ± SD, analysed by Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001

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