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Fig. 3 | Cell & Bioscience

Fig. 3

From: Defb19 regulates the migration of germ cell and is involved in male fertility

Fig. 3

DEFB19 acts as a chemoattractant for spermatocyte migration. a, b Representative images and corresponding quantification of wound healing assay comparing the closure rate of scratch area both in GC1-Spg (a) and GC2-Spd (b) treated with 400ng/ml rDEFB19 and vehicle control (n = 3 independent experiments). c, d Representative images and corresponding quantitative analyses of transwell migration assay comparing the number of GC1-Spg and GC2-Spd (n = 8 independent experiments) migrating through the transwell in response to 400 ng/ml rDEFB19 treatment or vehicle control. e Schematic diagram showing the setup of GC1-spg/GC2-Spd and 15P-1 co-cultured transwell migration assay. f Representative images showing the migrated GC1-spg co-cultured with Defb19-overexpressed or vector control-transduced 15P-1 lines. Corresponding quantitative analyses is shown on the bottom panel (n = 3 independent experiments). g Representative images showing the migrated GC2-spd after co-cultured with Defb19-overexpressed or vector control-transduced 15P-1 lines. Corresponding quantitative analyses of transwell migration assay is shown on the right (n = 3 independent experiments). Data are presented as means ± SD, analyzed by Student’s t test. ** p < 0.01

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