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Fig. 2 | Cell & Bioscience

Fig. 2

From: Defb19 regulates the migration of germ cell and is involved in male fertility

Fig. 2

Overexpression of Defb19 alters cell adhesion and motility of Sertoli cell. a, b RT-qPCR analysis (a) and representative immunofluorescence staining images (b) comparing the expression of Defb19 in Defb19- or vector control-transduced 15P-1 Sertoli cell line. DEFB19 was marked by green. DAPI (blue) marks cell nuclei. Scale bar = 20 μm. c Representative Western blot result of ES proteins (E-Cadherin - CDH1, N-Cadherin – CDH2 and β-Catenin – CTNNB1) and TJ proteins (ZO-1, Occludin - OCLD, Claudin3 – CLDN3, and Claudin11 – CLDN11) in Defb19-overexpressed and vector control transduced 15P-1 lines. GAPDH was used as loading control. d Quantification of Western blot results (n = 3 independent experiments). (e) Representative phase contrast images of adhesion assay showing the attachment of indicated 15P-1 lines on fibronectin (FN) at indicated time points. Attached cells were marked by red dashed line. f MTS assay quantifying the number of attached cells in adhesion assay (e, n = 3 independent experiments). g Representative time-lapse imaging images showing the migration of indicated 15P-1 lines at indicated time points. Red bars indicate the cell boundaries. h Quantitation of the migration rate of 15P-1 cells in the time-lapse migration assay at indicated time points (n = 3 independent experiments). Data are presented as means ± SD, analyzed by Student’s t test (AC). * p < 0.05, ** p < 0.01

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