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Fig. 1 | Cell & Bioscience

Fig. 1

From: Defb19 regulates the migration of germ cell and is involved in male fertility

Fig. 1

The expression profile of Defb19 in the male reproductive tract of mice. a RT-qPCR analysis comparing the expression of Defb19 in the indicated stages of spermatogenesis (n = 3 mice each with three technical replicates). GAPDH was used as internal control. b Representative images of frozen sections of testis immunofluorescence staining (n = 3 mice) showing the expression of DEFB19 (green) in germ cells and Sertoli cells. SOX9 (red) marks the nuclei of Sertoli cells, DAPI (blue) marks cell nuclei. Red arrowhead indicate spermatocyte, Yellow arrowhead indicate round spermatid, and white arrowhead is spermatid, respectively, Scale bar = 5 μm. c RT-qPCR analysis comparing the expression of Defb19 in three segments, caput, corpus and cauda of epididymis (n = 3 mice each with three technical replicates) and in matured sperm. GAPDH was used as internal control. d Representative immunofluorescence staining images of paraffin-embed epididymis section (n = 3 mice) showing the expression of DEFB19 (green), DAPI (blue) marks cell nuclei, Scale bar = 50 μm in the epididymis, scale bar = 10 μm in the sperm. Data are presented as means ± SD, analysed by One-way ANOVA combined Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01

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