Fig. 2
From: A synthetic pregnenolone analog promotes microtubule dynamics and neural development
An in vitro screening of P5 derivatives reveals compounds with microtubule polymerization activity similar to P5. A Detection of fluorescent microtubules produced by the in vitro microtubule polymerization assay under the TIRF microscopy. Scale bar 10 µm. B Screening of compounds that increase microtubule numbers. ***P < 0.001 when compared with the DMSO control (Ctr) by the Dunnett’s post-hoc test after one-way ANOVA analysis. The shaded areas represent numbers covering 1 or 2 standard deviations (σ) of P5 and DMSO, respectively. C Chemical structures of P5 and its derivatives. Relevant carbons in the P5 structure are labelled accordingly