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Fig. 7 | Cell & Bioscience

Fig. 7

From: Male guanine-rich RNA sequence binding factor 1 knockout mice (Grsf1−/−) gain less body weight during adolescence and adulthood

Fig. 7

Comparison of the testicular proteome of Grsf1−/− mice and wildtype controls. Total proteins were extracted from testes of three Grsf1−/− mice and three wildtype controls and the proteomes were quantified as described in the Materials and Methods section. A 5167 proteins were detected using mass spectrometry. Of these, 302 proteins were differentially regulated based on an unadjusted t-test p < 0.05 (blue and red). In red are those proteins with an absolute log2 fold-change > 1.5. Proteins below the p-value cut-off but with an absolute log2 fold-change > 1.5are shown in green. B Overrepresentation analysis of GO-terms. 302 differentially expressed proteins (unadjusted p-value) were used as input protein list. The background universe consisted of 5167 proteins detected using mass spectrometric analysis. The top five GO terms per category based on their gene ratio (k/n with k = genes of this cluster belonging to GO term and n = genes of all clusters belonging to GO term) are shown. Biological processes (BP) are depicted in yellow, cellular component (CC) in grey and molecular function in dark blue. C Hierarchically clustered heatmaps of regulated proteins (unadjusted p < 0.05 and log2 fold-change > 0.5) of associated with the GO term ‘mitochondrion’ (GO:0005739). Colours indicate z-score scaled protein expression values, with blue for low and red for high expression levels

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